Objective: |
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The project
concerns the traceability of origin and authenticity of olive oil by
genomic and metabolomic approaches. Starting from commercially
available cultivars a database will be constructed including all
relevant information for science, commerce, marketing and processing
in the EU. The project applies established DNA analytical methods
based on PCR (SSR, AFLP, RAPD, ITS, retrotransposons and SNP) and
metabolic profiling with mass spectrometry to develop molecular
markers for application in advanced high-throughput platform, mass
spectrometry, Light Cycler and microarrays which allow for a precise
discrimination and quantification of different cultivar composition in
olive oil of different origin and technological processing. Methods
will be validated for their application to forensic tests on olive
oils, enabling to understand their composition also in quantitative
terms and the presence of frauds. |